HOME학술지천식 및 알레르기

Title Measurement Specific IgE against Recombinant 16-kD and 19-kD Buckwheat Allergens for the Diagnosis of Buckwheat Allergy
Author Jae-Hyun Lee1, Yong Won Lee1, Yoo-Seob Shin1, Hae-Sim Park2, Chein-Soo Hong1 and Jung-Won Park1
Place of duty Department of Internal Medicine, 1Yonsei University College of Medicine, 2Ajou University College of Medicine, Seoul, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Buckwheat (BW, Fagopyrum esculentum)- specific IgE (sIgE) measurement is crucial to diagnose BW allergy. However, clinical application is difficult due to a high false-positive rate. Previously we identified 16-kD 2S storage albumin and 19-kD vicilin-like protein as BW major allergens. Objective: To investigate clinical usefulness of sIgE measurements against recombinant 16- and 19-kD BW allergens compared to the skin prick test and measurement of specific IgE against whole buckwheat (WBW) extract. Method: Eleven BW allergic patients, 11 asymptomatic sensitizers to BW and 16 non-atopic subjects were enrolled. We performed the skin prick test, sIgE measurement against recombinant 16- and 19-kD BW allergens and WBW extract using a CAP system. Result: In BW allergic patients, sIgE against WBW extract, r16- and r19-kD BW allergens were detected in 10, 5 and 9 patients, respectively. In asymptomatic sensitizers, sIgE against WBW extract, r16- and r19-kD BW allergens were detected in 9, 1 and 2 patients respectively. In ROC analysis, the AUC value of sIgE level was higher in r19-kD allergen than in r16-kD or WBW extract. Conclusion: We suggest that CAP sIgE against r19-kD and r16-kD BW allergens can be a diagnostic marker to discriminate BW allergic patients from asymptomatic sensitizers in a clinical setting. (Korean J Asthma Allergy Clin Immunol 2010;30:209-215)
Key_word Buckwheat, Allergy, Recombinant, Allergen, Specific IgE measurement
Publication info Korean J Asthma Allergy Clin Immunol 2010 Sep; 030(03): 209-215.
제 목 메밀 알레르기 진단에 있어서 16-kD 및 19-kD 재조합 알레르겐 특이 IgE 측정의 유용성
저 자 이재현1ㆍ이용원1ㆍ신유섭1ㆍ박해심2ㆍ홍천수1ㆍ박중원1
소 속 1연세대학교 의과대학 내과학교실, 2아주대학교 의과대학 내과학교실
출판정보 대한천식알레르기학회지 2010 Sep; 030(03): 209-215.