HOME학술지천식 및 알레르기

Title Proteomic Analysis of Airway Epithelial Cell Autoantigens Associated with Bronchial Asthma: Target Protein Identification Using Cultured Cell Fractionation Method
Author Hye-Ah Lee1, Yeon-Jeong Lee2, Byul Kwon1, Gil-Soon Choi1, Han-Jung Park1, Young-Min Ye1, Hae-Sim Park1, Yup Kang2 and Dong-Ho Nahm1
Place of duty 1Department of Allergy and Rheumatology, 2The Laboratory of Endocrinology, Ajou University School of Medicine, Suwon, Korea.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Involvement of the autoimmune mechanism has been suggested for the pathogenesis of bronchial asthma and several autoantigens which are associated with bronchial asthma have been identified. Objective: To identify novel asthma-associated autoantigens by proteomic analysis of proteins which are extracted by the cultured cell fractionation method according to the physical affinity of airway epithelial cells (A549) to the culture matrix. Method: Autoantigens were screened with serum samples from patients with severe asthma and the target autoantigens were identified by mass spectrometry. Result: Cultured A549 cells were differentially fractionated into easily detachable floating cell fraction and firmly attached cell fraction. Easily detachable cell fractioncontaining senescent cell and necrotic cell debris had many detectable asthma-related autoantigens when screened by immunoblot analysis using IgG antibodies from patients with severe asthma. In mass spectrometry analysis of 95-kDa autoantigen, ailpha-actin 4 was identified as a new airway epithelial autoantigen which was recognized by IgG autoantibody from a patient with severe asthma. This was reconfirmed by using specific antibody. Conclusion: Proteomic analysis of the airway epithelial cells using the differential fractionation method according to the physical characteristics of cultured airway epithelial cells may be a novel method for the identification of additional asthma- related autoantigens. (Korean J Asthma Allergy Clin Immunol 2008;28:192-198)
Key_word Asthma, Proteomics, Mass spectrometry, Autoantibodies, Autoantigens
Publication info Korean J Asthma Allergy Clin Immunol 2008 Sep; 028(03): 192-198.
제 목 기관지천식과 관련된 기도상피세포 자가항원의 단백체 분석: 세포배양 분획을 활용한 표적단백질의 규명 방법
저 자 이혜아1ㆍ이연정2ㆍ권별1ㆍ최길순1ㆍ박한정1ㆍ예영민1ㆍ박해심1ㆍ강엽2ㆍ남동호1
소 속 아주대학교 의과대학 1알레르기-류마티스 내과, 2내분비연구실
출판정보 대한천식및알레르기학회지 2008 Sep; 028(03): 192-198.